Functional potential of sewage sludge digestate microbes to degrade aliphatic hydrocarbons during bioremediation of a petroleum hydrocarbons contaminated soil

Sewage sludge digestate is a valuable organic waste which can be used as fertilizer in soil bioremediation. not only good source of nutrients but also rich bacteria carrying alkB genes, are involved aliphatic hydrocarbons metabolism. Increase genes ratio polluted soils has been observed to improve bioremediation efficiency. In this study, for the first time, genetic potential indigenous microorganisms degrade petroleum products was assessed. The objectives were study (PHCs) removal together with shifts taxa and changes concentration after application. Initial contaminated 1.5% 4.5%, respectively. During incubation digestate, percentage increased up 11.5% addition immobilized onto biochar value 60%. Application positively affected respiration bacterial density, concomitant enhanced PHCs degradation. Incubation amended resulted 74% decrease 2 months, while extra 95%. use microbial community profiles by increasing initial density diversity, including containing recognized degraders. This reveals great amendment additionally improves abundance soils.